Black mould lesions were caused by Alternaria alternata in 76% of 228 tomato fruit with characteristic sunken black lesions collected
from fields of processing tomatoes in California. Analysis of 29 RAPD primers revealed a high level of genetic diversity among the
69 isolates tested. Two major phenetic groups (Group 1 with 55 isolates and Group 2 with 14) were identified independently by
PCA and by UPGMA of Jaccard similarity coefficients. Only 34 of 137 RAPD markers were monomorphic for all isolates and the
genetic similarity between the two groups was 50%. Co-infection of black mould lesions by genetically distinct strains of A. alternata
occurred in two of 10 isolates tested. There was no evidence for geographic clustering of isolates with high levels of genetic
similarity, suggesting that isolates are widely dispersed across California. Only one isolate was identified which also caused stem
canker disease on a susceptible tomato cv., suggesting that these strains play a minor role in causing black mould on processing
tomatoes in California. This isolate and two other known stem canker isolates were clustered together with 11 other isolates in
Group 2. Group 2-specific bands were also identified in a survey of seven additional isolates known to produce host-specific toxins.